Building and Transferring a Successful Immunoassay

Improve Your ELISA Workflow Quality and Reproducibility

Whether you’re running an immunoassay to discover the next generation of therapeutics, developing a clinical test to track an emerging infectious disease, keeping our food supply safe or developing new vaccines, the accuracy and reproducibility of your immunoassay data is critical. With numerous assay steps, reagents, dispensing and detection tools involved, keeping CVs low and getting reproducible data can be a challenge. Additionally, transferability of your immunoassay from one group to another from discovery through clinical stage must be considered when the assay is constructed. Artel can provide the tools and expertise including industry guidance, ISO requirements and share best practices compiled by working with the leading biopharmaceutical labs during the past 35 years and is able to significantly enhance the productivity of your lab. Here are just a few ways that Artel can help.

The Artel MVS® (Multichannel Verification System) can assist understanding and managing variability in each of the parameters in your Assay.

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Speed up ELISA development & optimization

How many washes are optimal? Are the mixing conditions effective? Are the automated liquid handler (ALH) transfers accurate? Is every tip delivering the specified volume to each well? Which ELISA steps are most sensitive to liquid handling variability depending on the liquid class of the reagents dispensed? And is there operator variability?

Approximately 40% of ELISA assays fail at some point during the assay’s lifetime if these parameters are not managed and well understood. The MVS can help develop the test plan and protocol faster and with more confidence addressing the needs of the lab. Whether you are using a manual multichannel pipette or an ALH, you can use the MVS to test different washing and mixing conditions and reagent transfers quickly and efficiently. By using the MVS to assess mixing, washing and reagent dispense steps, you can get to optimal conditions much faster, thus saving time, reagents, and money.

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Troubleshoot ELISAs faster

Do you experience problems with reproducibility, high backgrounds, large CVs, or your standard curve? The MVS can help with these as well by enabling efficient assessment of a number of factors including:

  • Pipetting accuracy (manual and ALH)
  • Pipetting technique
  • Assay conditions such as various reagent classes (aqueous, glycerin, DMSO), washing and mixing
  • Assay consumable variability such as tips and microplates

For steps involving single channel pipettes, a PCS (Pipette Calibration System) can be used to quickly and easily assess these factors.

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Artel MVS Multichannel Verification System

Artel MVS

Do you know if your automated liquid handler volumes are on target, run after run?

Verify volume transfer precision and accuracy in your lab and simplify regulatory compliance with the easy-to-use MVS.

Verify proper pipetting technique

Are some technologists and scientists in the lab able to run ELISAs with smaller CVs than others? It might be because they have better pipetting technique. Proper pipetting is an often-underappreciated skill, but aspirating too fast or slow, placing the tip too high or low or at an angle, and even pausing too long between aspirating and dispensing can decrease pipetting accuracy and increase variability. And as these problems with pipetting technique propagate across a multistep procedure like an ELISA, the CVs in the final data can be negatively impacted.

The MVS (for multichannel pipettes) and Artel PCS® (Pipette Calibration System) (for single channel pipettes) can help here as well, by enabling quick checks of a scientist’s pipetting technique to ensure that this is not increasing the variability of your ELISA results. Additionally, scientists can attain Artel’s training programs conducted at our lab or at the customers site.

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Easily ensure accurate and precise volume transfers

And, of course, the MVS and PCS are great tools to ensure that your pipettes are performing as expected by making it easy to perform weekly, monthly, quarterly, semi-annual, or annual volume verification checks as needed for your regulated or non-regulated lab.

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Artel PCS Pipette Calibration System

The best way to calibrate pipettes – and the only way to ensure operator competency.

Are your pipettes and operators delivering accurate and reproducible results, every time? Ensure your pipettes and operators are performing properly.

Get extra support with Artel Services, Training and Consulting Support

Need expert help with any of these tasks? Artel offers a complete suite of consulting services including automated liquid handler calibration, assay optimization support, proper pipette technique assessment, industry and ISO guidance, training, and more.

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Artel liquid handling experts are here to help you.